Author: ccrosby (Page 3 of 5)

Chengyi Tu, a fourth-year graduate student in the Zoldan Lab, has passed his thesis proposal. He proposes to investigate how reactive oxygen species and the PI3k pathway direct stem cell fate, with a detailed focus on cardiac stem cell differentiation. Congratulations Chengyi!

Chengyi, with his new orca, ROS!

Elissa Baron (working with Alicia Allen) and David Shu (working with Cody Crosby) received undergraduate research fellowships (URFs), which includes a stipend of $1000.00 to aid their research. From the UT Office of the Vice President for Research:

The Undergraduate Research Fellowship (URF) program provides support for specific scholarly research projects conducted by full-time UT undergraduate students enrolled in any department. These fellowships are intended to cover costs associated with academic research projects proposed and written by student applicants and undertaken with the supervision of a university tenured or tenure-track faculty member, lecturer, senior lecturer or full-time research scientist/engineer.

The Zoldan Lab congratulates Elissa Barone, who presented her research on fiber alignment and its effect on cardiomyocyte differentiation at the Undergraduate Research Symposium in December 2016. Elissa is currently working with Alicia Allen.

Krista Polansky, a sophomore who is working with Julie Strickland, received an undergraduate research fellowship. She proposes to use this fellowship to isolate the individual progenitor cell populations found in murine neurospheres.

We’re happy to announce that Julie Strickland, a second-year in the Zoldan Lab, passed her qualifying exam! She discussed her plan for developing a hydrogel platform to rescue neurons in the brain.

Congrats to Jorge Gomez Medellin , who recently won an URF for his research (under Alicia Allen) on aligning cardiomyocytes on electro-spun scaffolds!

On April 4th, Dr. Zoldan attended a meeting of tissue engineers and material scientists in Bethseda, MD hosted by the National Eye Institute to assist in the development of retina organoid technology and set appropriate benchmarks for progress in the field.  Recent publications have highlighted the significance of creating 3D optic cups that mimic developmental process in humans.

On April 7th, Dr. Zoldan invited Dr. Steven Abrams, chair of pediatrics at Dell Medical School, to present at her seminar series “The Doctor is in the House”. Dr. Abrams gave an energizing talk “Global Childhood Malnutrition: Engineering Solutions for Human Problems” to a packed seminar room. Throughout the talk, Dr. Abrams emphasized the need for engineers and doctors to collaborate on important unsolved engineering problems, ranging from incorporating rare metals into the diets of malnourished children to re-designing the milk dispensers of premature infants.

The Zoldan Lab welcomes Christine Wei, a junior at UT Austin who will be helping Cody Crosby and Julie Strickland with the development of their hydrogel-liposome system.

I wanted to join the Zoldan Lab, because of its work with stem cell research. This research has so much potential, and even the smallest amount of progress in understanding these cells will be revolutionary in the medical field. In this lab, I hope to better understand how stem cells function and react, and to help work toward developing medical applications that can help patients in the future- Christine Wei

Congratulations to Chengyi Tu, who published his work “Monitoring protein synthesis in live cancer cells” in the journal Integrative Biology. The abstract is shown below:

Protein synthesis is generally under sophisticated and dynamic regulation to meet the ever-changing demands of a cell. Global up or down-regulation of protein synthesis and the shift of protein synthesis location (as shown, for example, during cellular stress or viral infection) are recognized as cellular responses to environmental changes such as nutrient/oxygen deprivation or to alterations such as pathological mutations in cancer cells. Monitoring protein synthesis in single live cells can be a powerful tool for cancer research. Here we employed a microfluidic platform to perform high throughput delivery of fluorescent labeled tRNAs into multiple myeloma cells with high transfection efficiency (∼45%) and high viability (>80%). We show that the delivered tRNAs were actively recruited to the ER for protein synthesis and that treatment with puromycin effectively disrupted this process. Interestingly, we observed the scattered distribution of tRNAs in cells undergoing mitosis, which has not been previously reported. Fluorescence lifetime analysis detected extensive FRET signals generated from tRNAs labeled as FRET pairs, further confirming that the delivered tRNAs were used by active ribosomes for protein translation. Our work demonstrates that the microfluidic delivery of FRET labeled tRNAs into living cancer cells can provide new insights into basic cancer metabolism and has the potential to serve as a platform for drug screening, diagnostics, or personalized medication.